Paralemmin 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12035R-BF350-100uL |
| Product Name | Paralemmin 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Paralemmin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | KIAA0270; PALM; PALM_HUMAN; Paralemmin; Paralemmin1;Paralemmin-1. |
| Background | Paralemmin, also called Paralemmin-1 or PALM, is a widely expressed peripheral membrane protein that is involved in cell structure and shape. A hydrophobic protein, Paralemmin is anchored to the cytoplasmic side of the cell membrane via di-palmitoylation and prenylation of its C-terminal cysteine cluster. Functioning at the synapse to regulate neuronal plasticity and plasma membrane dynamics, Paralemmin can bind to the dopamine receptor D3, thereby reducing D3 expression and subsequent adenylate cyclase activity. Overexpression of Paralemmin induces fibroblasts to extend long filopodia and to assume extreme cell shapes, suggesting involvement in the formation and stabilization of the plasma membrane. Two isoforms of Paralemmin exists due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Paralemmin 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12035R-BF350) validated for, and at what dilution?
bs-12035R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12035R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Paralemmin 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12035R-BF350)?
KLH conjugated synthetic peptide derived from human Paralemmin 1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-12035R-BF350)?
AbBy Fluor-350 on bs-12035R-BF350: excitation / emission 346nm/442nm.