UNCX Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12043R-BF594-100uL |
| Product Name | UNCX Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UNCX |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Homeobox protein unc-4 homolog; Homeobox protein Uncx4.1; tcag7.1272; UNC homeobox; Unc4.1 homeobox; UNC4_HUMAN; Uncx; UNCX4.1. |
| Background | Members of the paired homeobox family play a role in regulating cell development and pattern formation during embryonic stages. UNCX (UNC homeobox), also known as UNCX4.1, is a 531 amino acid nuclear transcription factor involved in neurogenesis and somitogenesis. Containing one homeobox DNA-binding domain, UNCX belongs to the paired homeobox family and UNC4 subfamily. UNCX assists in the formation of connections between hypothalamic neurons and the pituitary, which is necessary for central neurons to deliver hormones into peripheral blood. UNCX also plays a role in maintaining differentiation of the axial skeleton and acts upstream of Pax-9. The gene encoding UNCX maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is UNCX Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12043R-BF594) validated for, and at what dilution?
bs-12043R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UNCX Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12043R-BF594) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Chicken.
How should bs-12043R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UNCX Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12043R-BF594)?
KLH conjugated synthetic peptide derived from human UNCX
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-12043R-BF594)?
AbBy Fluor-594 on bs-12043R-BF594: excitation / emission 590nm/617nm.