5HT1D Receptor Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12046R-APC-Cy7-100uL |
| Product Name | 5HT1D Receptor Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 5HT1D Receptor/SR-1D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3352 |
| Swiss Prot | P28221 |
| Subcellular location | Cell membrane |
| Synonyms | 5-hydroxytryptamine receptor 1D, 5-HT-1D, 5-HT1D, Serotonin 1D alpha receptor, 5-HT-1D-alpha, Serotonin receptor 1D |
| Background | The members of the G-protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. b-adrenergic bound to adrenaline activates adenylyl cyclase, while a2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the a2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories, designated SR-17 (5HT17). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is 5HT1D Receptor Polyclonal Antibody, APC-Cy7 Conjugated (bs-12046R-APC-Cy7) validated for, and at what dilution?
bs-12046R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5HT1D Receptor Polyclonal Antibody, APC-Cy7 Conjugated (bs-12046R-APC-Cy7) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rabbit.
How should bs-12046R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5HT1D Receptor Polyclonal Antibody, APC-Cy7 Conjugated (bs-12046R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human 5HT1D Receptor/SR-1D
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-12046R-APC-Cy7)?
APC-Cy7 on bs-12046R-APC-Cy7: excitation / emission 650nm/780nm.