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5HT3A Receptor Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-12051R-BF555-100uL
Product Name 5HT3A Receptor Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 5HT3A Receptor/SR-3A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3359
Swiss Prot P46098
Subcellular location Cell membrane
Synonyms HTR3; 5HT3R; 5-HT-3; 5-HT3A; 5-HT3R; 5-hydroxytryptamine receptor 3A; 5-HT3-A; 5-hydroxytryptamine receptor 3; Serotonin receptor 3A; Serotonin-gated ion channel receptor; HTR3A
Background This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is 5HT3A Receptor Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12051R-BF555) validated for, and at what dilution?

bs-12051R-BF555 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 5HT3A Receptor Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12051R-BF555) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse.

How should bs-12051R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 5HT3A Receptor Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12051R-BF555)?

KLH conjugated synthetic peptide derived from human 5HT3A Receptor/SR-3A

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-12051R-BF555)?

AbBy Fluor-555 on bs-12051R-BF555: excitation / emission 553nm/568nm.

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