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5HT4 Receptor Polyclonal Antibody, PE-Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • GuineaPig
Overview
Catalog # bs-12054R-PE-Cy5.5-100uL
Product Name 5HT4 Receptor Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, GuineaPig
Specifications
Conjugation PE-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 5HT4 Receptor
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3360
Swiss Prot Q13639
Subcellular location Cytoplasm, Cell membrane
Synonyms 5 HT 4; 5 HT4; 5 HT4R; 5 hydroxytryptamine 4 receptor; 5 hydroxytryptamine receptor 4; 5 hydroxytryptamine serotonin receptor 4; 5 hydroxytryptamine4 receptor; 5-HT-4; 5-ht4; 5-HT4R; 5H4; 5HT 4; 5HT4; 5ht4 receptor; 5HT4R; Cardiac 5 HT4 receptor; HTR 4; HTR4; serotonin 4 receptor; Serotonin 5 HT4 receptor; serotonin 5-ht-4 receptor; serotonin 5-ht-4a. Serotonin receptor 4; 5HT4R_HUMAN.
Background This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is 5HT4 Receptor Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-12054R-PE-CY5-5) validated for, and at what dilution?

BS-12054R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 5HT4 Receptor Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-12054R-PE-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, GuineaPig.

How should BS-12054R-PE-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 5HT4 Receptor Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-12054R-PE-CY5-5)?

KLH conjugated synthetic peptide derived from human 5HT4 Receptor

What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-12054R-PE-CY5-5)?

PE-Cy5.5 on BS-12054R-PE-CY5-5: excitation / emission 488nm/694nm.

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