P2Y10 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12070R-Cy5-100uL |
| Product Name | P2Y10 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human P2Y10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27334 |
| Swiss Prot | O00398 |
| Subcellular location | Cell membrane |
| Synonyms | G protein coupled purinergic receptor P2Y10; P2ry10; P2Y like receptor; P2Y purinoceptor 10; P2Y-like receptor; P2Y10; P2Y10_HUMAN; Purinergic receptor P2Y G protein coupled 10; Putative P2Y purinoceptor 10. |
| Background | Nucleotides are important extracellular signaling molecules that mediate several events, such as cell proliferation, differentiation, chemotaxis and cytokine release. The P2 receptor family is activated by the binding of nucleotides and is divided into two subfamilies, designated P2X and P2Y. The P2Y receptor family are G protein-coupled receptors that mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C (PLC). To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. P2Y10 (purinergic receptor P2Y, G-protein coupled, 10), also known as P2RY10, is a 339 amino acid multi-pass membrane protein that is thought to act as a receptor for purines coupled to G-proteins. P2Y10 is found at low levels in blood leukocytes and is upregulated during promyelocytic cell differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is P2Y10 Polyclonal Antibody, Cy5 Conjugated (bs-12070R-Cy5) validated for, and at what dilution?
bs-12070R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does P2Y10 Polyclonal Antibody, Cy5 Conjugated (bs-12070R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Horse, Rabbit.
How should bs-12070R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise P2Y10 Polyclonal Antibody, Cy5 Conjugated (bs-12070R-Cy5)?
KLH conjugated synthetic peptide derived from human P2Y10
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12070R-Cy5)?
Cy5 on bs-12070R-Cy5: excitation / emission 625,650nm/670nm.