P2Y11 Polyclonal Antibody

$Tissue\/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Incubation: Anti-P2Y11 Polyclonal Antibody, conjugated (bs-12071R-A488) 1:200, 1.5 hours at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.$

Tissue\/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Incubation: Anti-P2Y11 Polyclonal Antibody, conjugated (bs-12071R-A488) 1:200, 1.5 hours at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Cow
Pig
Horse

Overview
Catalog #	bs-12071R
Product Name	P2Y11 Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human P2Y11
Immunogen Range	71-170/374
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5032
Swiss Prot	Q96G91
Subcellular location	Cell membrane
Synonyms	P2RY11; P2Y purinoceptor 11; P2Y11; P2Y11 receptor; P2Y11_HUMAN; Purinergic receptor P2Y G protein coupled 11; Purinergic receptor P2Y11.
Background	P2Y purinoceptor 11 (P2Y11) is a 374 amino acid protein belonging to the G-protein coupled receptor one family. P2Y11 is a multi-pass cell membrane protein that acts as a receptor for both ATP and ATD coupled to G proteins. Due to these interactions, P2Y11 is involved in phosphatidylinositol-calcium and adenylyl cyclase pathways. Induced by DMSO and retinoic acid, P2Y11 is highly expressed in spleen tissue. A putative trans-splicing event involving the gene that encodes P2Y11 and an upstream gene encoding PPAN has been found to result in a fusion protein, designated PPAN-P2RY11.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200