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DNER Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
Overview
Catalog # bs-12115R-RBITC-100uL
Product Name DNER Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DNER
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Cell membrane
Synonyms Brain EGF repeat-containing transmembrane protein; Bret; Delta and Notch-like epidermal growth factor-related receptor; Delta/notch-like EGF-related receptor; Dner; DNER_HUMAN; PRO299; Transmembrane protein Bet; UNQ26.
Background Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DNER Polyclonal Antibody, RBITC Conjugated (bs-12115R-RBITC) validated for, and at what dilution?

bs-12115R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-12115R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DNER Polyclonal Antibody, RBITC Conjugated (bs-12115R-RBITC)?

KLH conjugated synthetic peptide derived from human DNER

What are the excitation and emission wavelengths of the RBITC conjugate (bs-12115R-RBITC)?

RBITC on bs-12115R-RBITC: excitation / emission 540nm/625nm.

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