Chromogranin C Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12120R-BF647-100uL |
| Product Name | Chromogranin C Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Chromogranin C/Sg II |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | CHGC; Chromogranin-C; MEN1; SCG2; SCG2_HUMAN; Secretogranin II; Secretogranin-2; Secretoneurin; Secretoneurin included; SgII; SN. |
| Background | Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A); chromogranin B (Chr-B), also known as secretogranin I; chromogranin C (also known as secretogranin II or Sg II); and secretogranin III (Sg III or SCG3). High levels of Chr-A expression are characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Chromogranin C Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12120R-BF647) validated for, and at what dilution?
bs-12120R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Chromogranin C Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12120R-BF647) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Horse.
How should bs-12120R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Chromogranin C Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12120R-BF647)?
KLH conjugated synthetic peptide derived from human Chromogranin C/Sg II
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12120R-BF647)?
AbBy Fluor-647 on bs-12120R-BF647: excitation / emission 650nm/665nm.