CART Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12145R-BF594-100uL |
| Product Name | CART Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CART |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | CART; CART prepropeptide; Cocaine and amphetamine regulated transcript; CART_HUMAN. |
| Background | The CART gene encodes for a protein which has an important role in the regulation of appetite and body weight. The CART (cocaine- and amphetamine-regulated transcript) neuropeptide is an mRNA that changes in response to psychostimulant drug administration. Injection of CART peptides into the ventral tegmental area produces psychostimulant-like effects. CART localizes to areas of the central and peripheral nervous systems and is involved in feeding behavior when injected centrally. Expression of CART in the rat hypothalamus is modulated by nutritional status, and injection of synthetic CART peptide into the forebrain ventricular system suppresses food intake, indicating a possible role in hypothalamic control of energy homeostasis. Its identification in cell bodies and central terminals of vagal afferent neurons additionally suggests a role in brainstem mechanisms of meal termination and satiety. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CART Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12145R-BF594) validated for, and at what dilution?
bs-12145R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12145R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CART Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12145R-BF594)?
KLH conjugated synthetic peptide derived from human CART
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-12145R-BF594)?
AbBy Fluor-594 on bs-12145R-BF594: excitation / emission 590nm/617nm.