ZIC5 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12147R-APC-Cy7-100uL |
| Product Name | ZIC5 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZIC5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | zic5; ZIC5_HUMAN; Zinc finger protein of the cerebellum 5; Zinc finger protein ZIC 5. |
| Background | Zic5 (zinc finger protein of the cerebellum 5) is a C2H2 zinc finger transcription factor that influences development of the neural crest. Zic family members are abundant in developing and adult cerebellum. Zic family members are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. Zic5 is closely linked to Zic2, a related family member on chromosome 13. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZIC5 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12147R-APC-Cy7) validated for, and at what dilution?
bs-12147R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12147R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZIC5 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12147R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human ZIC5
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-12147R-APC-Cy7)?
APC-Cy7 on bs-12147R-APC-Cy7: excitation / emission 650nm/780nm.