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ZNF312 Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-12148R-BF594-100uL
Product Name ZNF312 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZNF312
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55079
Swiss Prot Q8TBJ5
Subcellular location Nucleus
Synonyms FEZ; TOF; FEZL; FKSG36; ZFP312; ZNF312; Fez family zinc finger protein 2; Forebrain embryonic zinc finger-like protein 2; Zinc finger protein 312; Zinc finger protein Fez-like; FEZF2
Background Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. May be involved in innate immunity (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ZNF312 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12148R-BF594) validated for, and at what dilution?

bs-12148R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZNF312 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12148R-BF594) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Horse, Rabbit.

How should bs-12148R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZNF312 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12148R-BF594)?

KLH conjugated synthetic peptide derived from human ZNF312

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-12148R-BF594)?

AbBy Fluor-594 on bs-12148R-BF594: excitation / emission 590nm/617nm.