ZNF312 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12148R-Cy3-100uL |
| Product Name | ZNF312 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZNF312 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55079 |
| Swiss Prot | Q8TBJ5 |
| Subcellular location | Nucleus |
| Synonyms | FEZ; TOF; FEZL; FKSG36; ZFP312; ZNF312; Fez family zinc finger protein 2; Forebrain embryonic zinc finger-like protein 2; Zinc finger protein 312; Zinc finger protein Fez-like; FEZF2 |
| Background | Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. May be involved in innate immunity (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZNF312 Polyclonal Antibody, Cy3 Conjugated (bs-12148R-Cy3) validated for, and at what dilution?
bs-12148R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNF312 Polyclonal Antibody, Cy3 Conjugated (bs-12148R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Horse, Rabbit.
How should bs-12148R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNF312 Polyclonal Antibody, Cy3 Conjugated (bs-12148R-Cy3)?
KLH conjugated synthetic peptide derived from human ZNF312
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12148R-Cy3)?
Cy3 on bs-12148R-Cy3: excitation / emission 512,550nm/570,615nm.