DBX2 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12149R-BF750-100uL |
| Product Name | DBX2 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DBX2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 440097 |
| Subcellular location | Nucleus |
| Synonyms | Dbx 2; developing brain homeobox 2; FLJ16139; hlx3; DBX2_HUMAN. |
| Background | DBX2 is a 339 amino acid member of the H2.0 homeobox family. DBX2, which is localized to the nucleus, contains one homeobox DNA-binding domain, a region of 60 amino acids that binds DNA through a helix-turn-helix type of structure. DBX2, which is expressed in the forebrain, midbrain, hindbrain and spinal cord, has been implicated in CNS development.Specifically, DBX2 has been shown to play a role in spinal cord dorsal/ventral patterning, as well as the regionalization of the CNS. DBX2 is also thought to play a role in the production of multiple spinal cord cell types. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DBX2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12149R-BF750) validated for, and at what dilution?
bs-12149R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DBX2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12149R-BF750) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Horse, Chicken.
How should bs-12149R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DBX2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12149R-BF750)?
KLH conjugated synthetic peptide derived from human DBX2
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-12149R-BF750)?
AbBy Fluor-750 on bs-12149R-BF750: excitation / emission 749nm/775nm.