PDGFAA + PDGFBB Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12157R-BF750-100uL |
| Product Name | PDGFAA + PDGFBB Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDGFAA + PDGFBB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | Becaplermin ;c sis ;c-sis ;FLJ12858 ;PDGF 1 ;PDGF 2 ;PDGF A ;PDGF A chain ;PDGF B ;PDGF B chain ;PDGF subunit B ;PDGF-2 ;Pdgfb ;PDGFB_HUMAN ;Platelet derived growth factor A chain precursor ;Platelet derived growth factor alpha polypeptide ;Platelet derived growth factor B chain precursor ;Platelet derived growth factor beta polypeptide simian sarcoma viral v-sis oncogene homolog ;Platelet derived growth factor beta polypeptide ;platelet-derived growth factor 2 ;Platelet-derived growth factor B chain ;Platelet-derived growth factor beta polypeptide ;Platelet-derived growth factor subunit B ;Platelet-derived growth factor, beta polypeptide oncogene SIS ;Proto-oncogene c-Sis ;SIS ;SSV ;v sis platelet derived growth factor beta polypeptide simian sarcoma viral oncogene homolog; |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PDGFAA + PDGFBB Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12157R-BF750) validated for, and at what dilution?
bs-12157R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDGFAA + PDGFBB Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12157R-BF750) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-12157R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDGFAA + PDGFBB Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12157R-BF750)?
KLH conjugated synthetic peptide derived from human PDGFAA + PDGFBB
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-12157R-BF750)?
AbBy Fluor-750 on bs-12157R-BF750: excitation / emission 749nm/775nm.