Calcyphosine 1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12161R-Cy5-100uL |
| Product Name | Calcyphosine 1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Calcyphosine 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Calcyphosin; Calcyphosine 1; Calcyphosine; CAPS; CAPS1; CAYP1_HUMAN; Thyroid protein p24. |
| Background | CAPS1 is a 189 amino acid cytoplasmic protein that contains four EF-hand domains, which serve as calcium-binding sites, and was first identified in canine thyroid. Interestingly, CAPS1 is much less abundant in humans than in canines. Synthesis and phosphorylation of CAPS1 is upregulated by cAMP-agonists in thyrocytes. CAPS1 likely functions in the regulation of ionic transport and may be involved in cross-signaling between cAMP and Ca(+2)-phophatidylinositol cascades. In addition to thyriod, CAPS1 is expressed in brain, salivary glands and lung. Expression of CAPS1 is increased in endometrial cancer and prognosis seems to be dependent on the level of CAPS1 expression, indicating that CAPS1 may be an appropriate prognostic marker for patient survival. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Calcyphosine 1 Polyclonal Antibody, Cy5 Conjugated (bs-12161R-Cy5) validated for, and at what dilution?
bs-12161R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Calcyphosine 1 Polyclonal Antibody, Cy5 Conjugated (bs-12161R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Rabbit.
How should bs-12161R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Calcyphosine 1 Polyclonal Antibody, Cy5 Conjugated (bs-12161R-Cy5)?
KLH conjugated synthetic peptide derived from human Calcyphosine 1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12161R-Cy5)?
Cy5 on bs-12161R-Cy5: excitation / emission 625,650nm/670nm.