KCNF1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12173R-100uL |
| Product Name | KCNF1 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KCNF1 |
| Immunogen Range | 161-260/494 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | IK 8; IK8; KCNF 1; KCNF; Kcnf1; KCNF1_HUMAN; kH 1; kH1; KV5.1; Potassium voltage-gated channel subfamily F member 1; MGC33316; Potassium channel KH1; Potassium channel Kv5.1; Potassium voltage gated channel subfamily F member 1; Potassium voltage-gated channel subfamily F member 1; Voltage gated potassium channel subunit Kv5.1; Voltage-gated potassium channel subunit Kv5.1. |
| Background | KCNF1 is a multi-pass membrane-bound protein that acts as an ion channel and is generally expressed as a heterotetramer of potassium channeling proteins. Formerly known as kH1, KCNF1 is usually found as a heteromer with three other potassium channel proteins, KCNG3, KV6.3 and KCNV2. As a potassium channel protein, KCNF1 plays a role in regulating apoptosis and proliferation of pulmonary artery smooth muscle (PASM) cells. Bone morphogenetic proteins (BMPs) restrict proliferation and can induce apoptosis in normal human PASM cells and will upregulate expression of KCNF1 in PASM cells in vitro. KCNF1 is expressed in heart, brain, liver, skeletal muscle, kidney and pancreas. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KCNF1 Polyclonal Antibody (bs-12173R) validated for, and at what dilution?
bs-12173R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KCNF1 Polyclonal Antibody (bs-12173R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-12173R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KCNF1 Polyclonal Antibody (bs-12173R)?
KLH conjugated synthetic peptide derived from human KCNF1 Immunogen range: 161-260/494.