phospho-KCNJ1 (Ser44) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12176R-BF750-100uL |
| Product Name | phospho-KCNJ1 (Ser44) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human KCNJ1 around the phosphorylation site of Ser44 |
| Modification Site | Ser44 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | KCNJ1 phospho S44; p-KCNJ1 phospho S44; KCNJ1 phospho Ser44; p-KCNJ1 Ser44; p-ROM-Kphospho S44; KCNJ1 phospho S25; p-KCNJ1 phospho S25; KCNJ1 phospho Ser25; p-KCNJ1 Ser25; p-ROM-Kphospho S25; ROM K; ROM-K; inwardly rectying subfamily J member 1; ATP regulated potassium channel ROM K; ATP sensitive inward rectier potassium channel 1; ATP-regulated potassium channel ROM-K; ATP-sensitive inward rectier potassium channel 1; Inward rectier K+ channel Kir1.1; inwardly rectying K+ channel; IRK1_HUMAN; KCNJ 1; KCNJ; Kcnj1; Kir 1.1; Kir1.1; Potassium channel; Potassium channel inwardly rectying subfamily J member 1; potassium inwardly-rectying channel J1; ROMK 1; ROMK 2; ROMK; ROMK1; ROMK2. |
| Background | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-KCNJ1 (Ser44) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12176R-BF750) validated for, and at what dilution?
bs-12176R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12176R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-KCNJ1 (Ser44) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12176R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human KCNJ1 around the phosphorylation site of Ser44
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-12176R-BF750)?
AbBy Fluor-750 on bs-12176R-BF750: excitation / emission 749nm/775nm.