phospho-KIR5.1 (Ser416) Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12180R-RBITC |
| Product Name | phospho-KIR5.1 (Ser416) Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human KIR5.1 around the phosphorylation site of Ser416 |
| Modification Site | Ser416 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | p-KIR5.1 phospho S416; 6430410F18Rik; AI132396; BIR9; Inward rectier K channel Kir5.1; Inward rectier K+ channel Kir5.1; Inward rectier potassium channel 16; IRK16; IRKG; KCNJ16; MGC33717; Potassium channel inwardly rectying subfamily J member 16; Potassium inwardly rectying channel subfamily J member 16; RP23-218O16.1; IRK16_HUMAN. |
| Background | The KIR family of potassium channels possess a greater tendency to allow potassium to flow into the cell rather than out of it. Kir4.1, also known as Kir1.2, is highly expressed in brain including glial cells, astrocytes and cortical neurons. Kir4.1 is also expressed in myelin-synthesizing oligodendrocytes and is crucial to myelination in the developing nervous system. The gene encoding human Kir4.1 maps to chromosome 1. Kir4.2, also known as Kir1.3, is expressed in kidney, lung, heart, thymus and thyroid during development. The gene encoding human Kir4.2 maps to chromosome 21 in the Down syndrome chromosome region 1, and Kir4.2 may play a role in the pathogenesis of Down?s syndrome. Kir 5.1 forms functional channels only by coexpression with either Kir4.1 or Kir4.2 in the kidney and pancreas. The gene encoding human Kir5.1 maps to chromosome 17. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-KIR5.1 (Ser416) Polyclonal Antibody, RBITC Conjugated (bs-12180R-RBITC) validated for, and at what dilution?
bs-12180R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12180R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-KIR5.1 (Ser416) Polyclonal Antibody, RBITC Conjugated (bs-12180R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human KIR5.1 around the phosphorylation site of Ser416
What are the excitation and emission wavelengths of the RBITC conjugate (bs-12180R-RBITC)?
RBITC on bs-12180R-RBITC: excitation / emission 540nm/625nm.