Kv2.2 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12186R-BF680 |
| Product Name | Kv2.2 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Kv2.2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | delayed rectier potassium channel protein; KCNB2; KCNB2_HUMAN; potassium channel Kv2.2; potassium voltage gated channel subfamily B member 2; Potassium voltage-gated channel subfamily B member 2; Voltage-gated potassium channel subunit Kv2.2. |
| Background | Voltage-gated K+ channels in the plasma membrane control the repolarization and the frequency of action potentials in neurons, muscles and other excitable cells. The KV gene family encodes more than 30 proteins that comprise the subunits of the K+ channels, and they vary in their gating and permeation properties, subcellular distribution and expression patterns. Functional KV channels assemble as tetramers consisting of pore-forming alpha subunits (KV), which include the KV1, KV2, KV3, KV4 and KV9 proteins, and accessory or KV-subunits that modify the gating properties of the coexpressed KV subunits. KV2.2 is a multi-pass membrane protein that regulates the voltage-dependent K+ permeability of excitable membranes. Its tail may be influential in the targeting of the channel to specific subcellular compartments and/or the regulation of channel activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Kv2.2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12186R-BF680) validated for, and at what dilution?
bs-12186R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Kv2.2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12186R-BF680) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-12186R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Kv2.2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12186R-BF680)?
KLH conjugated synthetic peptide derived from human Kv2.2
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-12186R-BF680)?
AbBy Fluor-680 on bs-12186R-BF680: excitation / emission 679nm/702nm.