GRP78 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1219R-BF647 |
| Product Name | GRP78 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GRP78 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3309 |
| Swiss Prot | P11021 |
| Subcellular location | Cytoplasm |
| Synonyms | BIP; MIF2; GRP78; HEL-S-89n; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 7 kDa protein 5; Immunoglobulin heavy chain-binding protein; HSPA5 |
| Background | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC1, probably to facilitate the release of DNAJC1 from its substrate. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GRP78 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1219R-BF647) validated for, and at what dilution?
bs-1219R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRP78 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1219R-BF647) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.
How should bs-1219R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRP78 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1219R-BF647)?
KLH conjugated synthetic peptide derived from human GRP78
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-1219R-BF647)?
AbBy Fluor-647 on bs-1219R-BF647: excitation / emission 650nm/665nm.