ZNF281 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12208R-Cy5 |
| Product Name | ZNF281 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZNF281 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23528 |
| Subcellular location | Nucleus |
| Synonyms | Zfp281; GC box binding zinc finger protein 1; GC-box-binding zinc finger protein 1; GZP1; Transcription factor ZBP-99; ZBP 99; ZBP99; zfp281; Zinc finger DNA binding protein 99; Zinc finger DNA-binding protein 99; Zinc Finger Protein 281; ZN281_HUMAN; ZNF281; ZNP 99; ZNP 99 transcription factor; ZNP99. |
| Background | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF281, also known as GC-box-binding zinc finger protein 1, ZBP-99 or ZNP-99 (zinc finger DNA-binding protein 99), is a zinc finger protein that belongs to the Kr_ppel C2H2-type zinc finger protein family. It is expressed ubiquitously at low levels with predominant expression in kidney, liver, lymphocytes and placenta. ZNF281 localizes to the nucleus and contains four C2H2-type zinc fingers. ZNF281 plays a role in repressing the transcription of a variety of genes including Gastrin and ODC (ornithine decarboxylase). In particular, ZNF281 functions by binding to the G-rich box in the enhancer region of the gene. Upon DNA damage, ZNF281 may become phosphorylated by Atm or ATR. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ZNF281 Polyclonal Antibody, Cy5 Conjugated (bs-12208R-Cy5) validated for, and at what dilution?
bs-12208R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNF281 Polyclonal Antibody, Cy5 Conjugated (bs-12208R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken.
How should bs-12208R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNF281 Polyclonal Antibody, Cy5 Conjugated (bs-12208R-Cy5)?
KLH conjugated synthetic peptide derived from human ZNF281
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12208R-Cy5)?
Cy5 on bs-12208R-Cy5: excitation / emission 625,650nm/670nm.