SPAG4L/SRG4 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-SRG4 Polyclonal Antibody, Unconjugated (bs-1220R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-SRG4 Polyclonal Antibody, Unconjugated (bs-1220R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti SRG4\/TSARG4 Polyclonal Antibody, Unconjugated (bs-1220R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.$

Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti SRG4\/TSARG4 Polyclonal Antibody, Unconjugated (bs-1220R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-1220R
Product Name	SPAG4L/SRG4 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TSARG4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	140732
Swiss Prot	Q8TC36
Subcellular location	Cytoplasm, Nucleus
Synonyms	SPAG4L; TSARG4; dJ726C3.1; SUN domain-containing protein 5; Sad1 and UNC84 domain-containing protein 5; Sperm-associated antigen 4-like protein; Testis and spermatogenesis-related gene 4 protein; SUN5
Background	Testis and spermatogenesis related gene 4 may play a role in the development of cryptorchidism, the failure of the testis to descend from the abdomen into the scrotum [RGD] [TISSUE SPECIFICITY] Testis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200