ZNF346 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNF346 Polyclonal Antibody, Unconjugated (bs-12235R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNF346 Polyclonal Antibody, Unconjugated (bs-12235R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Horse

Overview
Catalog #	bs-12235R
Product Name	ZNF346 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZNF346
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23567
Subcellular location	Cytoplasm, Nucleus
Synonyms	DKFZp547M223; Double stranded RNA binding zinc finger protein; Double stranded RNA binding zinc finger protein JAZ; JAZ; Just another zinc finger protein; Zfp346; Zinc finger protein 346; ZN346_HUMAN; Znf346.
Background	Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF346 (Zinc finger protein 346), also known as JAZ (Just another zinc finger protein), is a 294 amino acid protein that contains four matrin-type zinc fingers. The matrin-type zinc finger, which is very similar in structure to the classical DNA-binding C2H2 zinc finger, was first identified in the protein matrin-3. The matrin-type zinc finger has also been identified in several spliceosome RNA-binding proteins. Two isoforms exists due to alternate splicing events.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200