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ZNF346 Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-12235R-Cy3-100uL
Product Name ZNF346 Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZNF346
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23567
Subcellular location Cytoplasm, Nucleus
Synonyms DKFZp547M223; Double stranded RNA binding zinc finger protein; Double stranded RNA binding zinc finger protein JAZ; JAZ; Just another zinc finger protein; Zfp346; Zinc finger protein 346; ZN346_HUMAN; Znf346.
Background Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF346 (Zinc finger protein 346), also known as JAZ (Just another zinc finger protein), is a 294 amino acid protein that contains four matrin-type zinc fingers. The matrin-type zinc finger, which is very similar in structure to the classical DNA-binding C2H2 zinc finger, was first identified in the protein matrin-3. The matrin-type zinc finger has also been identified in several spliceosome RNA-binding proteins. Two isoforms exists due to alternate splicing events.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ZNF346 Polyclonal Antibody, Cy3 Conjugated (bs-12235R-Cy3) validated for, and at what dilution?

bs-12235R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZNF346 Polyclonal Antibody, Cy3 Conjugated (bs-12235R-Cy3) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse.

How should bs-12235R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZNF346 Polyclonal Antibody, Cy3 Conjugated (bs-12235R-Cy3)?

KLH conjugated synthetic peptide derived from human ZNF346

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12235R-Cy3)?

Cy3 on bs-12235R-Cy3: excitation / emission 512,550nm/570,615nm.

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