DAZL Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12245R-BF680 |
| Product Name | DAZL Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DAZL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1618 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DAZ homolog; DAZ like autosomal; DAZ-like autosomal; deleted in azoospermia-like; DAZH; DAZL 1; DAZL; DAZL_HUMAN; DAZL1; DAZLA; Deleted in azoospermia like 1; Deleted in azoospermia like; Deleted in azoospermia like autosomal; Deleted in azoospermia-like 1; Deleted in azoospermia-like; Germline specic RNA binding protein; MGC26406; Spermatogenesis gene on the Y like autosomal; SPGY like autosomal; SPGY-like-autosomal; SPGYLA; Tpx2. |
| Background | The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DAZL Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12245R-BF680) validated for, and at what dilution?
bs-12245R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DAZL Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12245R-BF680) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-12245R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DAZL Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12245R-BF680)?
KLH conjugated synthetic peptide derived from human DAZL
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-12245R-BF680)?
AbBy Fluor-680 on bs-12245R-BF680: excitation / emission 679nm/702nm.