Pumilio 2 Polyclonal Antibody, PE Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12246R-PE |
| Product Name | Pumilio 2 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pumilio 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23369 |
| Subcellular location | Cytoplasm |
| Synonyms | FLJ36528; KIAA0235; MGC138251; MGC138253; PUM 2; Pum2; PUM2; PUMH2; Pumilio Drosphila homolog 2; Pumilio homolog 2 Drosophila; Pumilio homolog 2; Pumilio2; PUM2_HUMAN. |
| Background | Pumilio 2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding mRNA targets. It supports proliferation and self-renewal of stem cells by regulating the translation of key transcripts. The Pumilio gene encodes proteins that are required for development of germ stem cells in one or both sexes. The Pumilio protein interacts with the human Nanos1 protein and this interaction may play a conserved role in germ cell development. Pumilio 2 is highly expressed in testis and ovary and at lower levels in brain, heart, kidney, liver, muscle, placenta, intestine and stomach. It is also expressed in stem cells, germ cells and in most fetal tissues. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Pumilio 2 Polyclonal Antibody, PE Conjugated (bs-12246R-PE) validated for, and at what dilution?
bs-12246R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12246R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Pumilio 2 Polyclonal Antibody, PE Conjugated (bs-12246R-PE)?
KLH conjugated synthetic peptide derived from human Pumilio 2
What are the excitation and emission wavelengths of the PE conjugate (bs-12246R-PE)?
PE on bs-12246R-PE: excitation / emission 496,564nm/578nm.