FUT2 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12258r-bf555 |
| Product Name | FUT2 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FUT2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Fucosyltransferase 2; Se; SE2; SEC2; Secretor factor;FUT2_HUMAN. |
| Background | Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. FUT2 is expressed on the surface of several human tumor cell lines such as BEL-7404, SPC-A-1, and SGC-7901. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is FUT2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12258R-BF555) validated for, and at what dilution?
bs-12258R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FUT2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12258R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-12258R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FUT2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12258R-BF555)?
KLH conjugated synthetic peptide derived from human FUT2
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-12258R-BF555)?
AbBy Fluor-555 on bs-12258R-BF555: excitation / emission 553nm/568nm.