GPA33 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1226R-Cy7-100uL |
| Product Name | GPA33 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse GPA33 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59290 |
| Swiss Prot | Q9JKA5 |
| Subcellular location | Cell membrane, Extracellular matrix |
| Synonyms | BB116197; 2010310L10Rik; 2210401D16Rik; Cell surface A33 antigen; Glycoprotein A33; mA33; Gpa33 |
| Background | The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319 amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213 amino acid extracellular region, a single transmembrane domain, and a 62 amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPA33 Polyclonal Antibody, Cy7 Conjugated (bs-1226R-Cy7) validated for, and at what dilution?
bs-1226R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPA33 Polyclonal Antibody, Cy7 Conjugated (bs-1226R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1226R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPA33 Polyclonal Antibody, Cy7 Conjugated (bs-1226R-Cy7)?
KLH conjugated synthetic peptide derived from mouse GPA33
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1226R-Cy7)?
Cy7 on bs-1226R-Cy7: excitation / emission 743nm/767nm.