JLP/MAX binding protein Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12270R-100uL |
| Product Name | JLP/MAX binding protein Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human JLP/JIP-4 |
| Immunogen Range | 101-188/1321 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 9043 |
| Swiss Prot | O60271 |
| Subcellular location | Cytoplasm |
| Synonyms | C jun amino terminal kinase interacting protein 4; c Jun NH2 terminal kinase associated leucine zipper protein; FLJ13450; FLJ14006; FLJ34602; HLC 4; HLC 4 protein; HLC 6; HLC4; HLC4 protein; HLC6; HSS; Human lung cancer protein 6; Human lung cancer protein 6; JIP 4; JIP 4; JIP4; JNK associated leucine zipper protein 1; JNK associated leucine zipper protein; JNK interacting protein 4; JNK interacting protein 4; JNK interacting protein; JNK/SAPK associated protein; KIAA0516; Lung cancer oncogene 4; MAPK8IP4; Max binding protein; MGC117291; MGC14967; MGC74461; Mitogen activated protein kinase 8 interacting protein 4; Mitogen activated protein kinase 8 interacting protein 4; PHET; PIG 6; PIG6; proliferation inducing gene 6; Protein highly expressed in testis; SPAG 9; SPAG9; Sperm associated antigen 9; Sperm specic protein; Sperm surface protein; Sunday driver drosophila homolog; Sunday driver 1; SYD1; JIP4_HUMAN. |
| Background | JIP-4 is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is JLP/MAX binding protein Polyclonal Antibody (bs-12270R) validated for, and at what dilution?
bs-12270R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JLP/MAX binding protein Polyclonal Antibody (bs-12270R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Sheep, Horse, Chicken.
How should bs-12270R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JLP/MAX binding protein Polyclonal Antibody (bs-12270R)?
KLH conjugated synthetic peptide derived from human JLP/JIP-4 Immunogen range: 101-188/1321.