MSY2 Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12271R-Biotin-100uL |
| Product Name | MSY2 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human YBOX2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 51087 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Contrin; CSDA 3; CSDA3; DBPC; DNA binding protein C; DNA-binding protein C; FRGY2 homolog; Germ cell specic Y box binding protein; Germ cell-specic Y-box-binding protein; MGC118270; MGC45104; MSY 2; MSY2; MSY2 homolog; OTTMUSP00000006276; RGD1305068; Y box binding protein 2; Y-box-binding protein 2; YBOX2_HUMAN; YBX 2; YBX2. |
| Background | MSY2 and YB-2 (MSY3,4) belong to the Y-box family of multifunctional proteins that regulate both transcription and translation (13). Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins (1). The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing arginine clusters and aromatic residues (4). MSY2 is expressed in testis and ovary where it may repress translation of parental mRNA (5,6). The gene encoding human MSY2 maps to chromosome 17p11.2-13.1 (5). YB-2 (MSY3,4 in mouse) is also known as DNA binding protein A and is highly expressed in the testis, heart and muscle (7,8). MSY2 and YB-2 bind to the consensus sequence 5'-UCCAUCA-3' contained in the Y-box element (9). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is MSY2 Polyclonal Antibody, Biotin Conjugated (bs-12271R-Biotin) validated for, and at what dilution?
bs-12271R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12271R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MSY2 Polyclonal Antibody, Biotin Conjugated (bs-12271R-Biotin)?
KLH conjugated synthetic peptide derived from human YBOX2