PREI3 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12275R-BF350-100uL |
| Product Name | PREI3 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PREI3/Phocein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25843 |
| Subcellular location | Cytoplasm |
| Synonyms | 2C4D; CGI 95; class II mMOB1; MGC12264; MOB family member 4, phocein; MOB like protein phocein; MOB1; mob1 homolog 3; MOB1, Mps One Binder kinase activator like 3; Mob3; MOB4; MOBKL3; MOBL3_HUMAN; mps one binder kinase activator like 3; Mps one binder kinase activator-like 3; Phocein Mob like protein; PHOCN; PREI3; Preimplantation protein 3; RCJMB04_1o21. |
| Background | Phocein is a 225 amino acid protein encoded by the human gene MOBKL3. Phocein belongs to the MOB1/phocein family and is phosphorylated on serine residues. Phocein is a widely expressed, highly conserved intracellular protein. The sequence of Phocein has limited homology to the sigma subunits from Clathrin adaptor complexes and contains an additional stretch bearing a putative SH3-binding domain. Phocein is usually associated with membranes but can be present in the cytosol, where it behaves as a protein complex. Phocein is the major partner of the striatin family members, which are scaffolding proteins involved in signaling and trafficking. Due to its association with Dynamin via direct interactions with nucleotide diphosphate kinase (NDPK) and Eps15, Phocein has been implicated in vesicular trafficking, acting in particular in the endocytic process. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PREI3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12275R-BF350) validated for, and at what dilution?
bs-12275R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12275R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PREI3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12275R-BF350)?
KLH conjugated synthetic peptide derived from human PREI3/Phocein
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-12275R-BF350)?
AbBy Fluor-350 on bs-12275R-BF350: excitation / emission 346nm/442nm.