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SOX7 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-12277R-Cy5
Product Name SOX7 Polyclonal Antibody, Cy5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SOX7
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 83595
Swiss Prot Q9BT81
Subcellular location Nucleus
Synonyms MGC10895; SOX 7; SOX 7 transcription factor; sox7; SOX7 transcription factor; SOX7_HUMAN; SRY sex determining region Y box 7; SRY box 7; SRY sex determining region Y box 7; Transcription factor SOX 7; Transcription factor Sox-7; Transcription factor SOX7.
Background Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SOX7 Polyclonal Antibody, Cy5 Conjugated (bs-12277R-Cy5) validated for, and at what dilution?

bs-12277R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SOX7 Polyclonal Antibody, Cy5 Conjugated (bs-12277R-Cy5) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Rabbit.

How should bs-12277R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SOX7 Polyclonal Antibody, Cy5 Conjugated (bs-12277R-Cy5)?

KLH conjugated synthetic peptide derived from human SOX7

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12277R-Cy5)?

Cy5 on bs-12277R-Cy5: excitation / emission 625,650nm/670nm.