Sohlh1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12278R-BF680-100uL |
| Product Name | Sohlh1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sohlh1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 402381 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | GM110; Helix Loop Helix Protein TOHLH1; Sohlh1; SOLH1_HUMAN; Spermatogenesis- and oogenesis-specic basic helix-loop-helix-containing protein 1; TOHLH1. |
| Background | SOHLH1 is a 328 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one bHLH domain through which it may function as a transcription factor during oogenesis and spermatogenesis. The gene encoding SOHLH1 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, and Familial dysautonomia, are both associated with chromosome 9. Notably, chromosome 9 encompasses the largest interferon family gene cluster. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Sohlh1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12278R-BF680) validated for, and at what dilution?
bs-12278R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sohlh1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12278R-BF680) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Horse.
How should bs-12278R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Sohlh1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12278R-BF680)?
KLH conjugated synthetic peptide derived from human Sohlh1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-12278R-BF680)?
AbBy Fluor-680 on bs-12278R-BF680: excitation / emission 679nm/702nm.