DDX3Y Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12286R-BF488-100uL |
| Product Name | DDX3Y Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DDX3Y |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8653 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DBY; DEAD Asp-Glu-Ala-Asp box polypeptide 3 Y linked; DEAD box protein 3 Y chromosomal; DEAD/H Asp-Glu-Ala-Asp/His box polypeptide Y chromosome; DDX3Y_HUMAN. |
| Background | DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DDX3Y Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12286R-BF488) validated for, and at what dilution?
bs-12286R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12286R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DDX3Y Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12286R-BF488)?
KLH conjugated synthetic peptide derived from human DDX3Y
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-12286R-BF488)?
AbBy Fluor-488 on bs-12286R-BF488: excitation / emission 499nm/519nm.