RBMY1F Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12290R-APC-100uL |
| Product Name | RBMY1F Polyclonal Antibody, APC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RBMY1F |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 159163 |
| Subcellular location | Nucleus |
| Synonyms | YRRM2; RNA binding mot protein, Y chromosome, family 1 member F/J; RNA binding mot protein, Y linked, family 1, member F; Y chromosome RNA recognition mot 2; YRRM2; RBY1F_HUMAN. |
| Background | The RBM (RNA-binding motif) gene family encodes proteins with an RNA binding motif. RBMY (RBM, Y chromosome) encodes a germ-cell specific nuclear protein involved in spermatogenesis. The RBM gene family, including RBMY1A, RBMY1B, RBMY1D, RBMY1E, RBMY1F, RBMY1H and RBMY1J, is comprised of at least 30 genes and pseudogenes, found on both arms of the Y chromosome. RBM X, an ancestral X chromosome homolog of the RBMY gene, encodes hnRNP G, which is widely expressed, whereas the RBMY gene evolved a male-specific function in spermatogenesis. Micro-deletions of the AZFb region of the Y chromosome, which contains a number of RBMY genes, usually result in severe consequences for spermatogenesis. RBM expression is localized to the nuclei of germ cells and RBM interacts with Tra2beta. Tra2beta is a ubiquitous activator of pre-mRNA splicing, but is most highly expressed in testis, suggesting a role for RBM in Tra2beta-dependent splicing in spermatocytes. The human RBMX gene maps to chromosome Xq26 and the RBMY gene family is found on all mammalian Y chomosomes. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RBMY1F Polyclonal Antibody, APC Conjugated (bs-12290R-APC) validated for, and at what dilution?
bs-12290R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RBMY1F Polyclonal Antibody, APC Conjugated (bs-12290R-APC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-12290R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RBMY1F Polyclonal Antibody, APC Conjugated (bs-12290R-APC)?
KLH conjugated synthetic peptide derived from human RBMY1F
What are the excitation and emission wavelengths of the APC conjugate (bs-12290R-APC)?
APC on bs-12290R-APC: excitation / emission 650nm/660nm.