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Hemogen/EDAG Polyclonal Antibody, Cy7 Conjugated


  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
Catalog # bs-12303R-Cy7
Product Name Hemogen/EDAG Polyclonal Antibody, Cy7 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity Human
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hemogen/EDAG
Immunogen Range 51-150/484
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID 55363
Subcellular location Nucleus
Synonyms EDAG; EDAG-1; Erythroid dferentiation-associated gene protein; HEMGN; HEMGN_HUMAN; Hemogen; Hemopoietic gene protein; Negative dferentiation regulator protein.
Background Hemogen is a 484 amino acid protein encoded by the human gene HEMGN. Hemogen is a nuclear protein that is expressed in hematopoietic precursor cells and can be detected in CD34+ and K-562 leukemia cell line. It is also expressed in bone marrow, testis, thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia. Hemogen is down-regulated during megakaryocytic differentiation of K-562 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) (at protein level). It can be up-regulated in normal PBMCs by mitogens.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200