Hemogen Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12303R-PE-Cy5.5-100uL |
| Product Name | Hemogen Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hemogen/EDAG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55363 |
| Subcellular location | Nucleus |
| Synonyms | EDAG; EDAG-1; Erythroid dferentiation-associated gene protein; HEMGN; HEMGN_HUMAN; Hemogen; Hemopoietic gene protein; Negative dferentiation regulator protein. |
| Background | Hemogen is a 484 amino acid protein encoded by the human gene HEMGN. Hemogen is a nuclear protein that is expressed in hematopoietic precursor cells and can be detected in CD34+ and K-562 leukemia cell line. It is also expressed in bone marrow, testis, thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia. Hemogen is down-regulated during megakaryocytic differentiation of K-562 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) (at protein level). It can be up-regulated in normal PBMCs by mitogens. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Hemogen Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-12303R-PE-CY5-5) validated for, and at what dilution?
BS-12303R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-12303R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hemogen Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-12303R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human Hemogen/EDAG
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-12303R-PE-CY5-5)?
PE-Cy5.5 on BS-12303R-PE-CY5-5: excitation / emission 488nm/694nm.