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PHEX Polyclonal Antibody, Cy5 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-12313R-Cy5-100uL
Product Name PHEX Polyclonal Antibody, Cy5 Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PHEX
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5251
Swiss Prot P78562
Subcellular location Cell membrane
Synonyms HYP; PEX; XLH; HPDR; HYP1; LXHR; HPDR1; Phosphate-regulating neutral endopeptidase; Metalloendopeptidase homolog PEX; Vitamin D-resistant hypophosphatemic rickets protein; X-linked hypophosphatemia protein; PHEX
Background Probably involved in bone and dentin mineralization and renal phosphate reabsorption.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PHEX Polyclonal Antibody, Cy5 Conjugated (bs-12313R-Cy5) validated for, and at what dilution?

bs-12313R-Cy5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PHEX Polyclonal Antibody, Cy5 Conjugated (bs-12313R-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-12313R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PHEX Polyclonal Antibody, Cy5 Conjugated (bs-12313R-Cy5)?

KLH conjugated synthetic peptide derived from human PHEX

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12313R-Cy5)?

Cy5 on bs-12313R-Cy5: excitation / emission 625,650nm/670nm.