NKX6.1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12314r-bf594 |
| Product Name | NKX6.1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Human NKX6.1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4825 |
| Synonyms | Homeobox protein NK-6 homolog A; Homeobox protein Nkx-6.1; NK homeo box, family 6, member A; NK homeobox Drosophila, family 6, A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1; NK6 transcription factor related, locus 1 Drosophila; NKX6-1; NKX6.1; NKX61; NKX61_HUMAN; NKX6A. |
| Background | Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature b cells, which produce insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic b cell specific genes, including insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in b cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is NKX6.1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12314R-BF594) validated for, and at what dilution?
bs-12314R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NKX6.1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12314R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-12314R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NKX6.1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12314R-BF594)?
KLH conjugated synthetic peptide derived from Human NKX6.1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-12314R-BF594)?
AbBy Fluor-594 on bs-12314R-BF594: excitation / emission 590nm/617nm.