GABRA1/GABA A Receptor alpha 1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1232R-Cy5.5-100uL |
| Product Name | GABRA1/GABA A Receptor alpha 1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GABRA1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2554 |
| Swiss Prot | P14867 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | EJM; ECA4; EJM5; EIEE19; Gamma-aminobutyric acid receptor subunit alpha-1; GABA(A) receptor subunit alpha-1; GABRA1 |
| Background | Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GABRA1/GABA A Receptor alpha 1 Polyclonal Antibody, Cy5.5 Conjugated (BS-1232R-CY5-5) validated for, and at what dilution?
BS-1232R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GABRA1/GABA A Receptor alpha 1 Polyclonal Antibody, Cy5.5 Conjugated (BS-1232R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Chicken.
How should BS-1232R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GABRA1/GABA A Receptor alpha 1 Polyclonal Antibody, Cy5.5 Conjugated (BS-1232R-CY5-5)?
KLH conjugated synthetic peptide derived from human GABRA1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-1232R-CY5-5)?
Cy5.5 on BS-1232R-CY5-5: excitation / emission 675nm/694nm.