L3MBTL3 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12342R-Cy3-100uL |
| Product Name | L3MBTL3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human L3MBTL3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84456 |
| Subcellular location | Nucleus |
| Synonyms | Hl3mbt like protein; KIAA1798; l3mbt like 3 Drosophila; l3mbt like 3; L3mbt like 3 protein; Lethal3malignant brain tumor like 3 protein; MBT1; LMBL3_HUMAN. |
| Background | Polycomb group (PcG) proteins are important for maintaining the transcriptionally repressed state of target genes and are thought to function via chromatin modification. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. PcG proteins are also required for normal maturation of myeloid progenitor cells. A putative PcG protein, L3MBTL3 (lethal(3)malignant brain tumor-like protein 3), also known as MBT-1 or KIAA1798, is a 780 amino acid protein containing three MBT repeats and one SAM (sterile alpha motif) domain. Localized to the nucleus, L3MBTL3 interacts with RING1B, another PcG protein that may be involved in the specification of anterior-posterior axis and cell proliferation in early development. L3MBTL3 exists as two isoforms produced by alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is L3MBTL3 Polyclonal Antibody, Cy3 Conjugated (bs-12342R-Cy3) validated for, and at what dilution?
bs-12342R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12342R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise L3MBTL3 Polyclonal Antibody, Cy3 Conjugated (bs-12342R-Cy3)?
KLH conjugated synthetic peptide derived from human L3MBTL3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12342R-Cy3)?
Cy3 on bs-12342R-Cy3: excitation / emission 512,550nm/570,615nm.