NCKAP1L Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12345R-Biotin-100uL |
| Product Name | NCKAP1L Polyclonal Antibody, Biotin Conjugated |
| Applications |
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCKAP1L/HEM1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 3071 |
| Subcellular location | Cell membrane |
| Synonyms | 4930568P13Rik; AI463083; HEM1; Hematopoietic protein 1; HEMATOPOIETIC PROTEIN HEM-1; Hemp1; Membrane associated protein hem1; Membrane-associated protein HEM-1; NCK associated protein 1 like; Nck-associated protein 1-like; NCKAP1L; NCKPL_HUMAN. |
| Background | HEM1 is a 1,127 amino acid single-pass membrane protein that localizes to the cytoplasmic side of the cell membrane. One of several members of the highly conserved HEM family of tissue-specific transmembrane proteins, HEM1 is expressed in cells of hematopoietic origin where it is thought to play an important role in oogenesis. The gene encoding HEM1 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is NCKAP1L Polyclonal Antibody, Biotin Conjugated (bs-12345R-Biotin) validated for, and at what dilution?
bs-12345R-Biotin is validated for IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12345R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCKAP1L Polyclonal Antibody, Biotin Conjugated (bs-12345R-Biotin)?
KLH conjugated synthetic peptide derived from human NCKAP1L/HEM1