SCXA Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-SCXA\/Scleraxis Polyclonal Antibody, Unconjugated (bs-2986R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-SCXA\/Scleraxis Polyclonal Antibody, Unconjugated (bs-2986R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat thyroid labeled with Anti-SCXA\/Scleraxis Polyclonal Antibody, Unconjugated (bs-12364R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat thyroid labeled with Anti-SCXA\/Scleraxis Polyclonal Antibody, Unconjugated (bs-12364R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Scleraxis Polyclonal Antibody, Unconjugated (bs-12364R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Scleraxis Polyclonal Antibody, Unconjugated (bs-12364R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Horse
Chicken

Overview
Catalog #	bs-12364r
Product Name	SCXA Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SCXA/Scleraxis
Immunogen Range	165-200/201
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	642658
Swiss Prot	Q7RTU7
Subcellular location	Nucleus
Synonyms	SCXA; SCXB; bHLHa48; Basic helix-loop-helix transcription factor scleraxis; Class A basic helix-loop-helix protein 41; bHLHa41; Class A basic helix-loop-helix protein 48; SCX
Background	Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200