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SCXA Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-12364R-BF350
Product Name SCXA Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Horse, Chicken
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SCXA/Scleraxis
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 642658
Swiss Prot Q7RTU7
Subcellular location Nucleus
Synonyms SCXA; SCXB; bHLHa48; Basic helix-loop-helix transcription factor scleraxis; Class A basic helix-loop-helix protein 41; bHLHa41; Class A basic helix-loop-helix protein 48; SCX
Background Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is SCXA Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12364R-BF350) validated for, and at what dilution?

bs-12364R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SCXA Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12364R-BF350) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse, Chicken.

How should bs-12364R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SCXA Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12364R-BF350)?

KLH conjugated synthetic peptide derived from human SCXA/Scleraxis

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-12364R-BF350)?

AbBy Fluor-350 on bs-12364R-BF350: excitation / emission 346nm/442nm.