MEGF10 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEGF10 Polyclonal Antibody, Unconjugated (bs-12372R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEGF10 Polyclonal Antibody, Unconjugated (bs-12372R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-12372R
Product Name	MEGF10 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MEGF10
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84466
Subcellular location	Cell membrane
Synonyms	MEG10_HUMAN; MEGF protein; megf10; Multiple EGF-like domains protein 10; multiple EGF-like-domains 10; Multiple epidermal growth factor-like domains 10; Multiple epidermal growth factor-like domains protein 10.
Background	MEGF10 is a 1,140 amino acid protein encoded by the human gene MEGF10. MEGF10 belongs to the MEGF family and contains fifteen EGF-like domains and one EMI domain. The engulfment of dying cells is a specialized form of phagocytosis that is highly conserved across evolution and is mediated by the concerted action of several molecules. MEGF10 is an engulfment receptor protein that localizes to the plasma membrane in a punctuated pattern. The MEGF10 protein shares structural similarities with nematode engulfment receptor cell death abnormal-1 (CED-1). MEGF10 function is modulated by the ATP binding cassette transporter ABCA1, which is an ortholog to CED-7. It is expressed at the cell surface in clusters around cell corpses during engulfment, and during the engulfment of apoptotic thymocytes is recruited to the bottom of the forming phagocytic cup.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200