HES2 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12383R-APC-Cy5.5-100uL |
| Product Name | HES2 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HES2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54626 |
| Subcellular location | Nucleus |
| Synonyms | bHLHb40; Class B basic helix loop helix protein 40; Hairy and enhancer of split 2 Drosophila; Transcription factor HES 2; HES2_HUMAN. |
| Background | The Drosophila hairy and Enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila hairy and Enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HES2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-12383R-APC-CY5-5) validated for, and at what dilution?
BS-12383R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HES2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-12383R-APC-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse.
How should BS-12383R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HES2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-12383R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human HES2
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-12383R-APC-CY5-5)?
APC-Cy5.5 on BS-12383R-APC-CY5-5: excitation / emission 650nm/694nm.