LMX1A Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12386r-bf647 |
| Product Name | LMX1A Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LMX1A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4009 |
| Synonyms | HGNC:6653; LIM homeobox protein 1.1; LIM homeobox transcription factor 1 alpha; LIM homeobox transcription factor 1-alpha; LIM/homeobox protein 1.1; LIM/homeobox protein LMX1A; LMX 1; LMX 1.1; LMX 1A; LMX-1.1; LMX1; LMX1.1; LMX1A; LMX1A_HUMAN; MGC87616. |
| Background | LMX1A belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. Two isoforms of LMX1A exist due to alternative splicing. Isoform 1 represents the full length protein and is expressed in many tissues including fetal brain, but is absent in heart, liver, spleen and testis. The second isoform, designated LMX1A-4AB, lacks amino acids 1-249 and is expressed in testis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is LMX1A Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12386R-BF647) validated for, and at what dilution?
bs-12386R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LMX1A Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12386R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-12386R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LMX1A Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12386R-BF647)?
KLH conjugated synthetic peptide derived from human LMX1A
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12386R-BF647)?
AbBy Fluor-647 on bs-12386R-BF647: excitation / emission 650nm/665nm.