Manic Fringe Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12390R-Biotin-100uL |
| Product Name | Manic Fringe Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Horse, Chimpanzee |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Manic Fringe/MFNG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 4242 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 3-N-acetylglucosaminyltransferase manic fringe; Beta-1; Beta-1,3-N-acetylglucosaminyltransferase manic fringe; MFNG; MFNG_HUMAN; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase. |
| Background | Three mammalian fringe family members, Manic, Radical and Lunatic Fringe, have been identified as proteins related to Drosophila fringe, a protein involved in development. Fringe proteins act upstream of the Notch signaling pathway and are involved in boundary determination during segmentation. Each mammalian fringe displays different patterns of expression, though all are expressed in the mouse embryo as well as in many adult tissues. Radical fringe plays a key role in the development of the limb bud. Lunatic fringe is required for normal somite segmentation and patterning and is thought to be a target of the molecular clock. Manic fringe, also involved in somatic development, has been shown to render mouse NIH/3T3 cells tumorigenic in SCID mice. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Manic Fringe Polyclonal Antibody, Biotin Conjugated (bs-12390R-Biotin) validated for, and at what dilution?
bs-12390R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12390R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Manic Fringe Polyclonal Antibody, Biotin Conjugated (bs-12390R-Biotin)?
KLH conjugated synthetic peptide derived from human Manic Fringe/MFNG