RBM15 Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12402R-Cy7-100uL |
| Product Name | RBM15 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RBM15 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | One twenty two protein; OTT; OTT1; SPEN; RNA binding mot protein 15; RBM15_HUMAN. |
| Background | RBM15 is a 977 amino acid protein that localizes to the nucleus and contains one SPOC domain and three RRM domains. Expressed as multiple alternatively spliced isoforms, RBM15 interacts with Epstein-Barr (EBV) viral proteins and is thought to be involved in the regulation of Hox genes, possibly via interactions with RNA and spliceosome components. RBM15 is subject to post-translational phosphorylation, probably by ATM or ATR. Chromosomal aberrations involving the RBM15 gene, which localizes to human chromosome 1, may be associated with the development of acute megakaryoblastic leukemia. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RBM15 Polyclonal Antibody, Cy7 Conjugated (bs-12402R-Cy7) validated for, and at what dilution?
bs-12402R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12402R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RBM15 Polyclonal Antibody, Cy7 Conjugated (bs-12402R-Cy7)?
KLH conjugated synthetic peptide derived from human RBM15
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-12402R-Cy7)?
Cy7 on bs-12402R-Cy7: excitation / emission 743nm/767nm.